Veteran Leander Paes and his partner Samantha Stosur suffered a second-round defeat in the mixed doubles event, ending India's campaign at the Australian Open on Tuesday.
The unseeded pair of Paes and Stosur won the first set 6-4 against Anna-Lena Groenefeld and Robert Farah but conceded the second set with the same scoreline.
After pulling out of Asian Games, Leander Paes dropped from Davis Cup World Group tie against Serbia
The third set went into tiebreak with the fifth seed German-Colombian duo emerging victorious 10-8.
Paes and Stosur had beaten the pair Dutch-Czech pairing of Wesley Koolhof Kveta Peschke in the round of 32.
The 45-year-old Paes had made a first round exit in the men's doubles event with partner Mexican Miguel Angel Reyes-Varela.
Jeevan Nedunchezhiyan and his partner Nicholas Monroe were also knocked out in the opening round of the event.
In the men's singles, Prajnesh Gunneswaran had also made a first round exit after coming through the Qualifiers while Ramkumar Ramanathan, Ankita Raina and Karman Thandi could not make it to the singles main draw.